Journal: BMC Neurology

Article Title: Nuclear entrapment and extracellular depletion of PCOLCE is associated with muscle degeneration in oculopharyngeal muscular dystrophy

doi: 10.1186/1471-2377-13-70

Figure Lengend Snippet: PCOLCE expression levels are down-regulated in OPMD patients and in a muscle cell model system. A . Plot shows expression levels of PCOLCE in biopsies from pre-symptomatic muscle that carry expPABPN1 but show no disease symptoms (N = 4) and OPMD symptomatic samples (N = 5). Fold change was calculated by reference to age-matched controls (N = 19). Only in the symptomatic group PCOLCE is significantly deregulated (*p < 0.05). B . RT-qPCR analysis of Pcolce mRNA levels in TA muscles of wild-type (FVB) and A17.1 OPMD model mice at 26-weeks of age. Fold change was calculated after normalisation to the Hprt housekeeping gene. Average scores are from 6 mice. Asterisk indicates significant decline (*p < 0.05). C . Pcolce levels in myotube cultures expressing expPABPN1-FLAG (Ala17) at a level similar to endogenous Pabpn1. (i) RT-qPCR analysis of Pcolce mRNA expression in myotube cultures of IM2 parental and Ala17 transgene containing cells. Fold change was calculated after normalisation to the Hprt housekeeping gene mRNA. Variations between fusion efficiencies were eliminated after normalisation to expression levels from the muscle specific actin ( Acta1 ) gene. Averages are from 3 biological replicates. Asterisk indicates significant decline (*p < 0.05). (ii) Western blot analysis of soluble protein extracts from unfused (-) and 4-day fused (+) IM2 and Ala17 cultures. The blot was incubated with anti-Pcolce antibody. Human PABPN1 is detected with an anti-FLAG antibody, the ACTA1 (MSA) protein is a marker for myotube differentiation and tubulin is used as a loading control.

Article Snippet: Primary antibodies used were: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich); mouse monoclonal anti-muscle actin (MSA) (Novocastra, Newcastle upon Tyne, UK); mouse monoclonal anti-α-Tubulin (Sigma-Aldrich); mouse anti-ubiquitin, FK2, (AbCam, Cambridge, UK); rabbit polyclonal anti-Pcolce (AbCam and Sigma-Aldrich); mouse anti-Dysferlin (Novocastra); anti-MBNL1 (donated by Dr. T.A.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Incubation, Marker

Journal: BMC Developmental Biology

Article Title: Apolipoprotein C-II and lipoprotein lipase show a temporal and geographic correlation with surfactant lipid synthesis in preparation for birth

doi: 10.1186/1471-213X-10-111

Figure Lengend Snippet: Distribution of PECAM-1 and α-sma proteins in the mouse fetal lung . Mouse tissue sections isolated on GD 17.5 were subjected to immunohistochemistry using anti-PECAM-1 (A, B) or anti-α-sma (C, D) as primary antibodies. Capillaries are PECAM-1-positive. Specific staining is clearly different between the two antibodies and only the staining pattern obtained with the anti-PECAM-1 corresponds to positive signals obtained with the anti-LPL antibody in Figure 2. Scale bars, 80 μm (A, C), 20 μm (B) or 40 μm (D). C , capillaries.

Article Snippet: A mouse anti-α-sma (VWR International CO., QC, Canada) (1:200) was also used.

Techniques: Isolation, Immunohistochemistry, Staining

Journal: Histochemistry and Cell Biology

Article Title: EBF1 is expressed in pericytes and contributes to pericyte cell commitment

doi: 10.1007/s00418-021-02015-7

Figure Lengend Snippet: Primary antibodies used in the study

Article Snippet: Mouse monoclonal anti-SMA , Biocare Medical , CM 001 A.

Techniques: